Measurement of image properties, e.g., region properties, contours.
Approximate a polygonal chain with the specified tolerance. | |
Downsample image by applying function | |
Compute a metric that indicates the strength of blur in an image (0 for no blur, 1 for maximal blur). | |
Return the (weighted) centroid of an image. | |
Calculate the Euler characteristic in binary image. | |
Find iso-valued contours in a 2D array for a given level value. | |
Test whether points on a specified grid are inside a polygon. | |
Compute the inertia tensor of the input image. | |
Compute the eigenvalues of the inertia tensor of the image. | |
Fraction of a channel's segmented binary mask that overlaps with a second channel's segmented binary mask. | |
Label connected regions of an integer array. | |
Manders' colocalization coefficient between two channels. | |
Manders' overlap coefficient | |
Marching cubes algorithm to find surfaces in 3d volumetric data. | |
Compute surface area, given vertices and triangular faces. | |
Calculate all raw image moments up to a certain order. | |
Calculate all central image moments up to a certain order. | |
Calculate all raw image moments up to a certain order. | |
Calculate all central image moments up to a certain order. | |
Calculate Hu's set of image moments (2D-only). | |
Calculate all normalized central image moments up to a certain order. | |
Calculate Pearson's Correlation Coefficient between pixel intensities in channels. | |
Calculate total perimeter of all objects in binary image. | |
Calculate total Crofton perimeter of all objects in binary image. | |
Test whether points lie inside a polygon. | |
Return the intensity profile of an image measured along a scan line. | |
Fit a model to data with the RANSAC (random sample consensus) algorithm. | |
Measure properties of labeled image regions. | |
Compute image properties and return them as a pandas-compatible table. | |
Calculate the Shannon entropy of an image. | |
Subdivision of polygonal curves using B-Splines. | |
Total least squares estimator for 2D circles. | |
Total least squares estimator for 2D ellipses. | |
Total least squares estimator for N-dimensional lines. |
skimage.measure.approximate_polygon(coords, tolerance) [source]
Approximate a polygonal chain with the specified tolerance.
It is based on the Douglas-Peucker algorithm.
Note that the approximated polygon is always within the convex hull of the original polygon.
coords(K, 2) array Coordinate array.
tolerancefloat Maximum distance from original points of polygon to approximated polygonal chain. If tolerance is 0, the original coordinate array is returned.
coords(L, 2) array Approximated polygonal chain where L <= K.
skimage.measure.block_reduce(image, block_size=2, func=<function sum>, cval=0, func_kwargs=None) [source]
Downsample image by applying function func to local blocks.
This function is useful for max and mean pooling, for example.
image(M[, …]) ndarray N-dimensional input image.
block_sizearray_like or int Array containing down-sampling integer factor along each axis. Default block_size is 2.
funccallable Function object which is used to calculate the return value for each local block. This function must implement an axis parameter. Primary functions are numpy.sum, numpy.min, numpy.max, numpy.mean and numpy.median. See also func_kwargs.
cvalfloat Constant padding value if image is not perfectly divisible by the block size.
func_kwargsdict Keyword arguments passed to func. Notably useful for passing dtype argument to np.mean. Takes dictionary of inputs, e.g.: func_kwargs={'dtype': np.float16}).
imagendarray Down-sampled image with same number of dimensions as input image.
>>> from skimage.measure import block_reduce
>>> image = np.arange(3*3*4).reshape(3, 3, 4)
>>> image
array([[[ 0, 1, 2, 3],
[ 4, 5, 6, 7],
[ 8, 9, 10, 11]],
[[12, 13, 14, 15],
[16, 17, 18, 19],
[20, 21, 22, 23]],
[[24, 25, 26, 27],
[28, 29, 30, 31],
[32, 33, 34, 35]]])
>>> block_reduce(image, block_size=(3, 3, 1), func=np.mean)
array([[[16., 17., 18., 19.]]])
>>> image_max1 = block_reduce(image, block_size=(1, 3, 4), func=np.max)
>>> image_max1
array([[[11]],
[[23]],
[[35]]])
>>> image_max2 = block_reduce(image, block_size=(3, 1, 4), func=np.max)
>>> image_max2
array([[[27],
[31],
[35]]])
skimage.measure.blur_effect(image, h_size=11, channel_axis=None, reduce_func=<function max>) [source]
Compute a metric that indicates the strength of blur in an image (0 for no blur, 1 for maximal blur).
imagendarray RGB or grayscale nD image. The input image is converted to grayscale before computing the blur metric.
h_sizeint, optional Size of the re-blurring filter.
channel_axisint or None, optional If None, the image is assumed to be grayscale (single-channel). Otherwise, this parameter indicates which axis of the array corresponds to color channels.
reduce_funccallable, optional Function used to calculate the aggregation of blur metrics along all axes. If set to None, the entire list is returned, where the i-th element is the blur metric along the i-th axis.
blurfloat (0 to 1) or list of floats Blur metric: by default, the maximum of blur metrics along all axes.
h_size must keep the same value in order to compare results between images. Most of the time, the default size (11) is enough. This means that the metric can clearly discriminate blur up to an average 11x11 filter; if blur is higher, the metric still gives good results but its values tend towards an asymptote.
Frederique Crete, Thierry Dolmiere, Patricia Ladret, and Marina Nicolas “The blur effect: perception and estimation with a new no-reference perceptual blur metric” Proc. SPIE 6492, Human Vision and Electronic Imaging XII, 64920I (2007) https://hal.archives-ouvertes.fr/hal-00232709 DOI:10.1117/12.702790
skimage.measure.centroid(image, *, spacing=None) [source]
Return the (weighted) centroid of an image.
imagearray The input image.
The pixel spacing along each axis of the image.
centertuple of float, length image.ndim The centroid of the (nonzero) pixels in image.
>>> image = np.zeros((20, 20), dtype=np.float64) >>> image[13:17, 13:17] = 0.5 >>> image[10:12, 10:12] = 1 >>> centroid(image) array([13.16666667, 13.16666667])
Use pixel graphs to find an object’s geodesic center
skimage.measure.euler_number(image, connectivity=None) [source]
Calculate the Euler characteristic in binary image.
For 2D objects, the Euler number is the number of objects minus the number of holes. For 3D objects, the Euler number is obtained as the number of objects plus the number of holes, minus the number of tunnels, or loops.
Input image. If image is not binary, all values greater than zero are considered as the object.
connectivityint, optional Maximum number of orthogonal hops to consider a pixel/voxel as a neighbor. Accepted values are ranging from 1 to input.ndim. If None, a full connectivity of input.ndim is used. 4 or 8 neighborhoods are defined for 2D images (connectivity 1 and 2, respectively). 6 or 26 neighborhoods are defined for 3D images, (connectivity 1 and 3, respectively). Connectivity 2 is not defined.
euler_numberint Euler characteristic of the set of all objects in the image.
The Euler characteristic is an integer number that describes the topology of the set of all objects in the input image. If object is 4-connected, then background is 8-connected, and conversely.
The computation of the Euler characteristic is based on an integral geometry formula in discretized space. In practice, a neighborhood configuration is constructed, and a LUT is applied for each configuration. The coefficients used are the ones of Ohser et al.
It can be useful to compute the Euler characteristic for several connectivities. A large relative difference between results for different connectivities suggests that the image resolution (with respect to the size of objects and holes) is too low.
S. Rivollier. Analyse d’image geometrique et morphometrique par diagrammes de forme et voisinages adaptatifs generaux. PhD thesis, 2010. Ecole Nationale Superieure des Mines de Saint-Etienne. https://tel.archives-ouvertes.fr/tel-00560838
Ohser J., Nagel W., Schladitz K. (2002) The Euler Number of Discretized Sets - On the Choice of Adjacency in Homogeneous Lattices. In: Mecke K., Stoyan D. (eds) Morphology of Condensed Matter. Lecture Notes in Physics, vol 600. Springer, Berlin, Heidelberg.
>>> import numpy as np >>> SAMPLE = np.zeros((100,100,100)); >>> SAMPLE[40:60, 40:60, 40:60]=1 >>> euler_number(SAMPLE) 1... >>> SAMPLE[45:55,45:55,45:55] = 0; >>> euler_number(SAMPLE) 2... >>> SAMPLE = np.array([[0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 1, 0, 0, 0, 0, 0], ... [0, 0, 0, 0, 0, 0, 0, 0, 1, 1, 1, 1, 0, 0, 0, 0, 0, 0], ... [0, 0, 0, 0, 0, 0, 0, 0, 1, 1, 1, 0, 0, 0, 0, 0, 0, 0], ... [0, 0, 0, 0, 0, 0, 0, 0, 1, 1, 1, 0, 0, 0, 0, 0, 0, 0], ... [0, 0, 0, 0, 0, 0, 1, 1, 1, 1, 1, 1, 0, 0, 0, 0, 0, 0], ... [0, 0, 0, 0, 0, 0, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 0], ... [0, 0, 0, 0, 0, 0, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 0], ... [1, 0, 1, 0, 0, 1, 1, 0, 1, 1, 0, 0, 1, 1, 1, 1, 1, 0], ... [0, 1, 1, 1, 1, 1, 1, 1, 0, 1, 1, 0, 0, 0, 1, 1, 1, 1], ... [0, 1, 1, 0, 0, 1, 1, 1, 0, 0, 0, 0, 0, 0, 0, 0, 1, 1]]) >>> euler_number(SAMPLE) # doctest: 0 >>> euler_number(SAMPLE, connectivity=1) # doctest: 2
skimage.measure.find_contours(image, level=None, fully_connected='low', positive_orientation='low', *, mask=None) [source]
Find iso-valued contours in a 2D array for a given level value.
Uses the “marching squares” method to compute the iso-valued contours of the input 2D array for a particular level value. Array values are linearly interpolated to provide better precision for the output contours.
image(M, N) ndarray of double Input image in which to find contours.
levelfloat, optional Value along which to find contours in the array. By default, the level is set to (max(image) + min(image)) / 2
Changed in version 0.18: This parameter is now optional.
fully_connectedstr, {‘low’, ‘high’} Indicates whether array elements below the given level value are to be considered fully-connected (and hence elements above the value will only be face connected), or vice-versa. (See notes below for details.)
positive_orientationstr, {‘low’, ‘high’} Indicates whether the output contours will produce positively-oriented polygons around islands of low- or high-valued elements. If ‘low’ then contours will wind counter-clockwise around elements below the iso-value. Alternately, this means that low-valued elements are always on the left of the contour. (See below for details.)
mask(M, N) ndarray of bool or None A boolean mask, True where we want to draw contours. Note that NaN values are always excluded from the considered region (mask is set to False wherever array is NaN).
contourslist of (K, 2) ndarrays Each contour is a ndarray of (row, column) coordinates along the contour.
See also
The marching squares algorithm is a special case of the marching cubes algorithm [1]. A simple explanation is available here:
https://users.polytech.unice.fr/~lingrand/MarchingCubes/algo.html
There is a single ambiguous case in the marching squares algorithm: when a given 2 x 2-element square has two high-valued and two low-valued elements, each pair diagonally adjacent. (Where high- and low-valued is with respect to the contour value sought.) In this case, either the high-valued elements can be ‘connected together’ via a thin isthmus that separates the low-valued elements, or vice-versa. When elements are connected together across a diagonal, they are considered ‘fully connected’ (also known as ‘face+vertex-connected’ or ‘8-connected’). Only high-valued or low-valued elements can be fully-connected, the other set will be considered as ‘face-connected’ or ‘4-connected’. By default, low-valued elements are considered fully-connected; this can be altered with the ‘fully_connected’ parameter.
Output contours are not guaranteed to be closed: contours which intersect the array edge or a masked-off region (either where mask is False or where array is NaN) will be left open. All other contours will be closed. (The closed-ness of a contours can be tested by checking whether the beginning point is the same as the end point.)
Contours are oriented. By default, array values lower than the contour value are to the left of the contour and values greater than the contour value are to the right. This means that contours will wind counter-clockwise (i.e. in ‘positive orientation’) around islands of low-valued pixels. This behavior can be altered with the ‘positive_orientation’ parameter.
The order of the contours in the output list is determined by the position of the smallest x,y (in lexicographical order) coordinate in the contour. This is a side effect of how the input array is traversed, but can be relied upon.
Warning
Array coordinates/values are assumed to refer to the center of the array element. Take a simple example input: [0, 1]. The interpolated position of 0.5 in this array is midway between the 0-element (at x=0) and the 1-element (at x=1), and thus would fall at x=0.5.
This means that to find reasonable contours, it is best to find contours midway between the expected “light” and “dark” values. In particular, given a binarized array, do not choose to find contours at the low or high value of the array. This will often yield degenerate contours, especially around structures that are a single array element wide. Instead, choose a middle value, as above.
Lorensen, William and Harvey E. Cline. Marching Cubes: A High Resolution 3D Surface Construction Algorithm. Computer Graphics (SIGGRAPH 87 Proceedings) 21(4) July 1987, p. 163-170). DOI:10.1145/37401.37422
>>> a = np.zeros((3, 3))
>>> a[0, 0] = 1
>>> a
array([[1., 0., 0.],
[0., 0., 0.],
[0., 0., 0.]])
>>> find_contours(a, 0.5)
[array([[0. , 0.5],
[0.5, 0. ]])]
skimage.measure.grid_points_in_poly(shape, verts, binarize=True) [source]
Test whether points on a specified grid are inside a polygon.
For each (r, c) coordinate on a grid, i.e. (0, 0), (0, 1) etc., test whether that point lies inside a polygon.
You can control the output type with the binarize flag. Please refer to its documentation for further details.
shapetuple (M, N) Shape of the grid.
verts(V, 2) array Specify the V vertices of the polygon, sorted either clockwise or anti-clockwise. The first point may (but does not need to be) duplicated.
If True, the output of the function is a boolean mask. Otherwise, it is a labeled array. The labels are: O - outside, 1 - inside, 2 - vertex, 3 - edge.
mask(M, N) ndarray If binarize is True, the output is a boolean mask. True means the corresponding pixel falls inside the polygon. If binarize is False, the output is a labeled array, with pixels having a label between 0 and 3. The meaning of the values is: O - outside, 1 - inside, 2 - vertex, 3 - edge.
See also
skimage.measure.inertia_tensor(image, mu=None, *, spacing=None) [source]
Compute the inertia tensor of the input image.
imagearray The input image.
muarray, optional The pre-computed central moments of image. The inertia tensor computation requires the central moments of the image. If an application requires both the central moments and the inertia tensor (for example, skimage.measure.regionprops), then it is more efficient to pre-compute them and pass them to the inertia tensor call.
The pixel spacing along each axis of the image.
Tarray, shape (image.ndim, image.ndim) The inertia tensor of the input image. \(T_{i, j}\) contains the covariance of image intensity along axes \(i\) and \(j\).
Bernd Jähne. Spatio-Temporal Image Processing: Theory and Scientific Applications. (Chapter 8: Tensor Methods) Springer, 1993.
skimage.measure.inertia_tensor_eigvals(image, mu=None, T=None, *, spacing=None) [source]
Compute the eigenvalues of the inertia tensor of the image.
The inertia tensor measures covariance of the image intensity along the image axes. (See inertia_tensor.) The relative magnitude of the eigenvalues of the tensor is thus a measure of the elongation of a (bright) object in the image.
imagearray The input image.
muarray, optional The pre-computed central moments of image.
Tarray, shape (image.ndim, image.ndim) The pre-computed inertia tensor. If T is given, mu and image are ignored.
The pixel spacing along each axis of the image.
eigvalslist of float, length image.ndim The eigenvalues of the inertia tensor of image, in descending order.
Computing the eigenvalues requires the inertia tensor of the input image. This is much faster if the central moments (mu) are provided, or, alternatively, one can provide the inertia tensor (T) directly.
skimage.measure.intersection_coeff(image0_mask, image1_mask, mask=None) [source]
Fraction of a channel’s segmented binary mask that overlaps with a second channel’s segmented binary mask.
image0_mask(M, N) ndarray of dtype bool Image mask of channel A.
image1_mask(M, N) ndarray of dtype bool Image mask of channel B. Must have same dimensions as image0_mask.
mask(M, N) ndarray of dtype bool, optional Only image0_mask and image1_mask pixels within this region of interest mask are included in the calculation. Must have same dimensions as image0_mask.
Fraction of image0_mask that overlaps with image1_mask.
skimage.measure.label(label_image, background=None, return_num=False, connectivity=None) [source]
Label connected regions of an integer array.
Two pixels are connected when they are neighbors and have the same value. In 2D, they can be neighbors either in a 1- or 2-connected sense. The value refers to the maximum number of orthogonal hops to consider a pixel/voxel a neighbor:
1-connectivity 2-connectivity diagonal connection close-up
[ ] [ ] [ ] [ ] [ ]
| \ | / | <- hop 2
[ ]--[x]--[ ] [ ]--[x]--[ ] [x]--[ ]
| / | \ hop 1
[ ] [ ] [ ] [ ]
label_imagendarray of dtype int Image to label.
backgroundint, optional Consider all pixels with this value as background pixels, and label them as 0. By default, 0-valued pixels are considered as background pixels.
return_numbool, optional Whether to return the number of assigned labels.
connectivityint, optional Maximum number of orthogonal hops to consider a pixel/voxel as a neighbor. Accepted values are ranging from 1 to input.ndim. If None, a full connectivity of input.ndim is used.
labelsndarray of dtype int Labeled array, where all connected regions are assigned the same integer value.
numint, optional Number of labels, which equals the maximum label index and is only returned if return_num is True.
Christophe Fiorio and Jens Gustedt, “Two linear time Union-Find strategies for image processing”, Theoretical Computer Science 154 (1996), pp. 165-181.
Kensheng Wu, Ekow Otoo and Arie Shoshani, “Optimizing connected component labeling algorithms”, Paper LBNL-56864, 2005, Lawrence Berkeley National Laboratory (University of California), http://repositories.cdlib.org/lbnl/LBNL-56864
>>> import numpy as np >>> x = np.eye(3).astype(int) >>> print(x) [[1 0 0] [0 1 0] [0 0 1]] >>> print(label(x, connectivity=1)) [[1 0 0] [0 2 0] [0 0 3]] >>> print(label(x, connectivity=2)) [[1 0 0] [0 1 0] [0 0 1]] >>> print(label(x, background=-1)) [[1 2 2] [2 1 2] [2 2 1]] >>> x = np.array([[1, 0, 0], ... [1, 1, 5], ... [0, 0, 0]]) >>> print(label(x)) [[1 0 0] [1 1 2] [0 0 0]]
Explore and visualize region properties with pandas
skimage.measure.manders_coloc_coeff(image0, image1_mask, mask=None) [source]
Manders’ colocalization coefficient between two channels.
image0(M, N) ndarray Image of channel A. All pixel values should be non-negative.
image1_mask(M, N) ndarray of dtype bool Binary mask with segmented regions of interest in channel B. Must have same dimensions as image0.
mask(M, N) ndarray of dtype bool, optional Only image0 pixel values within this region of interest mask are included in the calculation. Must have same dimensions as image0.
mccfloat Manders’ colocalization coefficient.
Manders’ Colocalization Coefficient (MCC) is the fraction of total intensity of a certain channel (channel A) that is within the segmented region of a second channel (channel B) [1]. It ranges from 0 for no colocalisation to 1 for complete colocalization. It is also referred to as M1 and M2.
MCC is commonly used to measure the colocalization of a particular protein in a subceullar compartment. Typically a segmentation mask for channel B is generated by setting a threshold that the pixel values must be above to be included in the MCC calculation. In this implementation, the channel B mask is provided as the argument image1_mask, allowing the exact segmentation method to be decided by the user beforehand.
The implemented equation is:
\(A_i\) is the value of the \(i^{th}\) pixel in image0 \(A_{i,coloc} = A_i\) if \(Bmask_i > 0\) \(Bmask_i\) is the value of the \(i^{th}\) pixel in mask
MCC is sensitive to noise, with diffuse signal in the first channel inflating its value. Images should be processed to remove out of focus and background light before the MCC is calculated [2].
Manders, E.M.M., Verbeek, F.J. and Aten, J.A. (1993), Measurement of co-localization of objects in dual-colour confocal images. Journal of Microscopy, 169: 375-382. https://doi.org/10.1111/j.1365-2818.1993.tb03313.x https://imagej.net/media/manders.pdf
Dunn, K. W., Kamocka, M. M., & McDonald, J. H. (2011). A practical guide to evaluating colocalization in biological microscopy. American journal of physiology. Cell physiology, 300(4), C723–C742. https://doi.org/10.1152/ajpcell.00462.2010
skimage.measure.manders_overlap_coeff(image0, image1, mask=None) [source]
Manders’ overlap coefficient
image0(M, N) ndarray Image of channel A. All pixel values should be non-negative.
image1(M, N) ndarray Image of channel B. All pixel values should be non-negative. Must have same dimensions as image0
mask(M, N) ndarray of dtype bool, optional Only image0 and image1 pixel values within this region of interest mask are included in the calculation. Must have ♣same dimensions as image0.
Manders’ Overlap Coefficient of pixel intensities between the two images.
Manders’ Overlap Coefficient (MOC) is given by the equation [1]:
\(A_i\) is the value of the \(i^{th}\) pixel in image0 \(B_i\) is the value of the \(i^{th}\) pixel in image1
It ranges between 0 for no colocalization and 1 for complete colocalization of all pixels.
MOC does not take into account pixel intensities, just the fraction of pixels that have positive values for both channels[R2208c1a5d6e1-2]_ [3]. Its usefulness has been criticized as it changes in response to differences in both co-occurence and correlation and so a particular MOC value could indicate a wide range of colocalization patterns [4] [5].
Manders, E.M.M., Verbeek, F.J. and Aten, J.A. (1993), Measurement of co-localization of objects in dual-colour confocal images. Journal of Microscopy, 169: 375-382. https://doi.org/10.1111/j.1365-2818.1993.tb03313.x https://imagej.net/media/manders.pdf
Dunn, K. W., Kamocka, M. M., & McDonald, J. H. (2011). A practical guide to evaluating colocalization in biological microscopy. American journal of physiology. Cell physiology, 300(4), C723–C742. https://doi.org/10.1152/ajpcell.00462.2010
Bolte, S. and Cordelières, F.P. (2006), A guided tour into subcellular colocalization analysis in light microscopy. Journal of Microscopy, 224: 213-232. https://doi.org/10.1111/j.1365-2818.2006.01
Adler J, Parmryd I. (2010), Quantifying colocalization by correlation: the Pearson correlation coefficient is superior to the Mander’s overlap coefficient. Cytometry A. Aug;77(8):733-42.https://doi.org/10.1002/cyto.a.20896
Adler, J, Parmryd, I. Quantifying colocalization: The case for discarding the Manders overlap coefficient. Cytometry. 2021; 99: 910– 920. https://doi.org/10.1002/cyto.a.24336
skimage.measure.marching_cubes(volume, level=None, *, spacing=(1.0, 1.0, 1.0), gradient_direction='descent', step_size=1, allow_degenerate=True, method='lewiner', mask=None) [source]
Marching cubes algorithm to find surfaces in 3d volumetric data.
In contrast with Lorensen et al. approach [2], Lewiner et al. algorithm is faster, resolves ambiguities, and guarantees topologically correct results. Therefore, this algorithm generally a better choice.
volume(M, N, P) ndarray Input data volume to find isosurfaces. Will internally be converted to float32 if necessary.
levelfloat, optional Contour value to search for isosurfaces in volume. If not given or None, the average of the min and max of vol is used.
spacinglength-3 tuple of floats, optional Voxel spacing in spatial dimensions corresponding to numpy array indexing dimensions (M, N, P) as in volume.
gradient_directionstring, optional Controls if the mesh was generated from an isosurface with gradient descent toward objects of interest (the default), or the opposite, considering the left-hand rule. The two options are: * descent : Object was greater than exterior * ascent : Exterior was greater than object
step_sizeint, optional Step size in voxels. Default 1. Larger steps yield faster but coarser results. The result will always be topologically correct though.
allow_degeneratebool, optional Whether to allow degenerate (i.e. zero-area) triangles in the end-result. Default True. If False, degenerate triangles are removed, at the cost of making the algorithm slower.
Whether the method of Lewiner et al. or Lorensen et al. will be used.
mask(M, N, P) array, optional Boolean array. The marching cube algorithm will be computed only on True elements. This will save computational time when interfaces are located within certain region of the volume M, N, P-e.g. the top half of the cube-and also allow to compute finite surfaces-i.e. open surfaces that do not end at the border of the cube.
verts(V, 3) array Spatial coordinates for V unique mesh vertices. Coordinate order matches input volume (M, N, P). If allow_degenerate is set to True, then the presence of degenerate triangles in the mesh can make this array have duplicate vertices.
faces(F, 3) array Define triangular faces via referencing vertex indices from verts. This algorithm specifically outputs triangles, so each face has exactly three indices.
normals(V, 3) array The normal direction at each vertex, as calculated from the data.
values(V,) array Gives a measure for the maximum value of the data in the local region near each vertex. This can be used by visualization tools to apply a colormap to the mesh.
The algorithm [1] is an improved version of Chernyaev’s Marching Cubes 33 algorithm. It is an efficient algorithm that relies on heavy use of lookup tables to handle the many different cases, keeping the algorithm relatively easy. This implementation is written in Cython, ported from Lewiner’s C++ implementation.
To quantify the area of an isosurface generated by this algorithm, pass verts and faces to skimage.measure.mesh_surface_area.
Regarding visualization of algorithm output, to contour a volume named myvolume about the level 0.0, using the mayavi package:
>>>
>> from mayavi import mlab
>> verts, faces, _, _ = marching_cubes(myvolume, 0.0)
>> mlab.triangular_mesh([vert[0] for vert in verts],
[vert[1] for vert in verts],
[vert[2] for vert in verts],
faces)
>> mlab.show()
Similarly using the visvis package:
>>> >> import visvis as vv >> verts, faces, normals, values = marching_cubes(myvolume, 0.0) >> vv.mesh(np.fliplr(verts), faces, normals, values) >> vv.use().Run()
To reduce the number of triangles in the mesh for better performance, see this example using the mayavi package.
Thomas Lewiner, Helio Lopes, Antonio Wilson Vieira and Geovan Tavares. Efficient implementation of Marching Cubes’ cases with topological guarantees. Journal of Graphics Tools 8(2) pp. 1-15 (december 2003). DOI:10.1080/10867651.2003.10487582
Lorensen, William and Harvey E. Cline. Marching Cubes: A High Resolution 3D Surface Construction Algorithm. Computer Graphics (SIGGRAPH 87 Proceedings) 21(4) July 1987, p. 163-170). DOI:10.1145/37401.37422
skimage.measure.mesh_surface_area(verts, faces) [source]
Compute surface area, given vertices and triangular faces.
verts(V, 3) array of floats Array containing coordinates for V unique mesh vertices.
faces(F, 3) array of ints List of length-3 lists of integers, referencing vertex coordinates as provided in verts.
areafloat Surface area of mesh. Units now [coordinate units] ** 2.
See also
The arguments expected by this function are the first two outputs from skimage.measure.marching_cubes. For unit correct output, ensure correct spacing was passed to skimage.measure.marching_cubes.
This algorithm works properly only if the faces provided are all triangles.
skimage.measure.moments(image, order=3, *, spacing=None) [source]
Calculate all raw image moments up to a certain order.
M[0, 0].M[1, 0] / M[0, 0], M[0, 1] / M[0, 0]}.Note that raw moments are neither translation, scale nor rotation invariant.
image(N[, …]) double or uint8 array Rasterized shape as image.
orderint, optional Maximum order of moments. Default is 3.
The pixel spacing along each axis of the image.
m(order + 1, order + 1) array Raw image moments.
Wilhelm Burger, Mark Burge. Principles of Digital Image Processing: Core Algorithms. Springer-Verlag, London, 2009.
B. Jähne. Digital Image Processing. Springer-Verlag, Berlin-Heidelberg, 6. edition, 2005.
T. H. Reiss. Recognizing Planar Objects Using Invariant Image Features, from Lecture notes in computer science, p. 676. Springer, Berlin, 1993.
>>> image = np.zeros((20, 20), dtype=np.float64) >>> image[13:17, 13:17] = 1 >>> M = moments(image) >>> centroid = (M[1, 0] / M[0, 0], M[0, 1] / M[0, 0]) >>> centroid (14.5, 14.5)
skimage.measure.moments_central(image, center=None, order=3, *, spacing=None, **kwargs) [source]
Calculate all central image moments up to a certain order.
The center coordinates (cr, cc) can be calculated from the raw moments as: {M[1, 0] / M[0, 0], M[0, 1] / M[0, 0]}.
Note that central moments are translation invariant but not scale and rotation invariant.
image(N[, …]) double or uint8 array Rasterized shape as image.
centertuple of float, optional Coordinates of the image centroid. This will be computed if it is not provided.
orderint, optional The maximum order of moments computed.
The pixel spacing along each axis of the image.
mu(order + 1, order + 1) array Central image moments.
Wilhelm Burger, Mark Burge. Principles of Digital Image Processing: Core Algorithms. Springer-Verlag, London, 2009.
B. Jähne. Digital Image Processing. Springer-Verlag, Berlin-Heidelberg, 6. edition, 2005.
T. H. Reiss. Recognizing Planar Objects Using Invariant Image Features, from Lecture notes in computer science, p. 676. Springer, Berlin, 1993.
>>> image = np.zeros((20, 20), dtype=np.float64)
>>> image[13:17, 13:17] = 1
>>> M = moments(image)
>>> centroid = (M[1, 0] / M[0, 0], M[0, 1] / M[0, 0])
>>> moments_central(image, centroid)
array([[16., 0., 20., 0.],
[ 0., 0., 0., 0.],
[20., 0., 25., 0.],
[ 0., 0., 0., 0.]])
skimage.measure.moments_coords(coords, order=3) [source]
Calculate all raw image moments up to a certain order.
M[0, 0].M[1, 0] / M[0, 0], M[0, 1] / M[0, 0]}.Note that raw moments are neither translation, scale, nor rotation invariant.
coords(N, D) double or uint8 array Array of N points that describe an image of D dimensionality in Cartesian space.
orderint, optional Maximum order of moments. Default is 3.
M(order + 1, order + 1, …) array Raw image moments. (D dimensions)
Johannes Kilian. Simple Image Analysis By Moments. Durham University, version 0.2, Durham, 2001.
>>> coords = np.array([[row, col] ... for row in range(13, 17) ... for col in range(14, 18)], dtype=np.float64) >>> M = moments_coords(coords) >>> centroid = (M[1, 0] / M[0, 0], M[0, 1] / M[0, 0]) >>> centroid (14.5, 15.5)
skimage.measure.moments_coords_central(coords, center=None, order=3) [source]
Calculate all central image moments up to a certain order.
M[0, 0].M[1, 0] / M[0, 0], M[0, 1] / M[0, 0]}.Note that raw moments are neither translation, scale nor rotation invariant.
coords(N, D) double or uint8 array Array of N points that describe an image of D dimensionality in Cartesian space. A tuple of coordinates as returned by np.nonzero is also accepted as input.
centertuple of float, optional Coordinates of the image centroid. This will be computed if it is not provided.
orderint, optional Maximum order of moments. Default is 3.
Mc(order + 1, order + 1, …) array Central image moments. (D dimensions)
Johannes Kilian. Simple Image Analysis By Moments. Durham University, version 0.2, Durham, 2001.
>>> coords = np.array([[row, col]
... for row in range(13, 17)
... for col in range(14, 18)])
>>> moments_coords_central(coords)
array([[16., 0., 20., 0.],
[ 0., 0., 0., 0.],
[20., 0., 25., 0.],
[ 0., 0., 0., 0.]])
As seen above, for symmetric objects, odd-order moments (columns 1 and 3, rows 1 and 3) are zero when centered on the centroid, or center of mass, of the object (the default). If we break the symmetry by adding a new point, this no longer holds:
>>> coords2 = np.concatenate((coords, [[17, 17]]), axis=0)
>>> np.round(moments_coords_central(coords2),
... decimals=2)
array([[17. , 0. , 22.12, -2.49],
[ 0. , 3.53, 1.73, 7.4 ],
[25.88, 6.02, 36.63, 8.83],
[ 4.15, 19.17, 14.8 , 39.6 ]])
Image moments and central image moments are equivalent (by definition) when the center is (0, 0):
>>> np.allclose(moments_coords(coords), ... moments_coords_central(coords, (0, 0))) True
skimage.measure.moments_hu(nu) [source]
Calculate Hu’s set of image moments (2D-only).
Note that this set of moments is proved to be translation, scale and rotation invariant.
nu(M, M) array Normalized central image moments, where M must be >= 4.
nu(7,) array Hu’s set of image moments.
M. K. Hu, “Visual Pattern Recognition by Moment Invariants”, IRE Trans. Info. Theory, vol. IT-8, pp. 179-187, 1962
Wilhelm Burger, Mark Burge. Principles of Digital Image Processing: Core Algorithms. Springer-Verlag, London, 2009.
B. Jähne. Digital Image Processing. Springer-Verlag, Berlin-Heidelberg, 6. edition, 2005.
T. H. Reiss. Recognizing Planar Objects Using Invariant Image Features, from Lecture notes in computer science, p. 676. Springer, Berlin, 1993.
>>> image = np.zeros((20, 20), dtype=np.float64)
>>> image[13:17, 13:17] = 0.5
>>> image[10:12, 10:12] = 1
>>> mu = moments_central(image)
>>> nu = moments_normalized(mu)
>>> moments_hu(nu)
array([0.74537037, 0.35116598, 0.10404918, 0.04064421, 0.00264312,
0.02408546, 0. ])
skimage.measure.moments_normalized(mu, order=3, spacing=None) [source]
Calculate all normalized central image moments up to a certain order.
Note that normalized central moments are translation and scale invariant but not rotation invariant.
mu(M[, …], M) array Central image moments, where M must be greater than or equal to order.
orderint, optional Maximum order of moments. Default is 3.
The pixel spacing along each axis of the image.
nu(order + 1``[, ...], ``order + 1) array Normalized central image moments.
Wilhelm Burger, Mark Burge. Principles of Digital Image Processing: Core Algorithms. Springer-Verlag, London, 2009.
B. Jähne. Digital Image Processing. Springer-Verlag, Berlin-Heidelberg, 6. edition, 2005.
T. H. Reiss. Recognizing Planar Objects Using Invariant Image Features, from Lecture notes in computer science, p. 676. Springer, Berlin, 1993.
>>> image = np.zeros((20, 20), dtype=np.float64)
>>> image[13:17, 13:17] = 1
>>> m = moments(image)
>>> centroid = (m[0, 1] / m[0, 0], m[1, 0] / m[0, 0])
>>> mu = moments_central(image, centroid)
>>> moments_normalized(mu)
array([[ nan, nan, 0.078125 , 0. ],
[ nan, 0. , 0. , 0. ],
[0.078125 , 0. , 0.00610352, 0. ],
[0. , 0. , 0. , 0. ]])
skimage.measure.pearson_corr_coeff(image0, image1, mask=None) [source]
Calculate Pearson’s Correlation Coefficient between pixel intensities in channels.
image0(M, N) ndarray Image of channel A.
image1(M, N) ndarray Image of channel 2 to be correlated with channel B. Must have same dimensions as image0.
mask(M, N) ndarray of dtype bool, optional Only image0 and image1 pixels within this region of interest mask are included in the calculation. Must have same dimensions as image0.
pccfloat Pearson’s correlation coefficient of the pixel intensities between the two images, within the mask if provided.
p-valuefloat Two-tailed p-value.
Pearson’s Correlation Coefficient (PCC) measures the linear correlation between the pixel intensities of the two images. Its value ranges from -1 for perfect linear anti-correlation to +1 for perfect linear correlation. The calculation of the p-value assumes that the intensities of pixels in each input image are normally distributed.
Scipy’s implementation of Pearson’s correlation coefficient is used. Please refer to it for further information and caveats [1].
\(A_i\) is the value of the \(i^{th}\) pixel in image0 \(B_i\) is the value of the \(i^{th}\) pixel in image1, \(m_A_i\) is the mean of the pixel values in image0 \(m_B_i\) is the mean of the pixel values in image1
A low PCC value does not necessarily mean that there is no correlation between the two channel intensities, just that there is no linear correlation. You may wish to plot the pixel intensities of each of the two channels in a 2D scatterplot and use Spearman’s rank correlation if a non-linear correlation is visually identified [2]. Also consider if you are interested in correlation or co-occurence, in which case a method involving segmentation masks (e.g. MCC or intersection coefficient) may be more suitable [3] [4].
Providing the mask of only relevant sections of the image (e.g., cells, or particular cellular compartments) and removing noise is important as the PCC is sensitive to these measures [3] [4].
Dunn, K. W., Kamocka, M. M., & McDonald, J. H. (2011). A practical guide to evaluating colocalization in biological microscopy. American journal of physiology. Cell physiology, 300(4), C723–C742. https://doi.org/10.1152/ajpcell.00462.2010
Bolte, S. and Cordelières, F.P. (2006), A guided tour into subcellular colocalization analysis in light microscopy. Journal of Microscopy, 224: 213-232. https://doi.org/10.1111/j.1365-2818.2006.01706.x
skimage.measure.perimeter(image, neighborhood=4) [source]
Calculate total perimeter of all objects in binary image.
image(M, N) ndarray Binary input image.
neighborhood4 or 8, optional Neighborhood connectivity for border pixel determination. It is used to compute the contour. A higher neighborhood widens the border on which the perimeter is computed.
perimeterfloat Total perimeter of all objects in binary image.
K. Benkrid, D. Crookes. Design and FPGA Implementation of a Perimeter Estimator. The Queen’s University of Belfast. http://www.cs.qub.ac.uk/~d.crookes/webpubs/papers/perimeter.doc
>>> from skimage import data, util >>> from skimage.measure import label >>> # coins image (binary) >>> img_coins = data.coins() > 110 >>> # total perimeter of all objects in the image >>> perimeter(img_coins, neighborhood=4) 7796.867... >>> perimeter(img_coins, neighborhood=8) 8806.268...
skimage.measure.perimeter_crofton(image, directions=4) [source]
Calculate total Crofton perimeter of all objects in binary image.
image(M, N) ndarray Input image. If image is not binary, all values greater than zero are considered as the object.
directions2 or 4, optional Number of directions used to approximate the Crofton perimeter. By default, 4 is used: it should be more accurate than 2. Computation time is the same in both cases.
perimeterfloat Total perimeter of all objects in binary image.
This measure is based on Crofton formula [1], which is a measure from integral geometry. It is defined for general curve length evaluation via a double integral along all directions. In a discrete space, 2 or 4 directions give a quite good approximation, 4 being more accurate than 2 for more complex shapes.
Similar to perimeter(), this function returns an approximation of the perimeter in continuous space.
S. Rivollier. Analyse d’image geometrique et morphometrique par diagrammes de forme et voisinages adaptatifs generaux. PhD thesis, 2010. Ecole Nationale Superieure des Mines de Saint-Etienne. https://tel.archives-ouvertes.fr/tel-00560838
>>> from skimage import data, util >>> from skimage.measure import label >>> # coins image (binary) >>> img_coins = data.coins() > 110 >>> # total perimeter of all objects in the image >>> perimeter_crofton(img_coins, directions=2) 8144.578... >>> perimeter_crofton(img_coins, directions=4) 7837.077...
skimage.measure.points_in_poly(points, verts) [source]
Test whether points lie inside a polygon.
points(K, 2) array Input points, (x, y).
verts(L, 2) array Vertices of the polygon, sorted either clockwise or anti-clockwise. The first point may (but does not need to be) duplicated.
mask(K,) array of bool True if corresponding point is inside the polygon.
See also
skimage.measure.profile_line(image, src, dst, linewidth=1, order=None, mode='reflect', cval=0.0, *, reduce_func=<function mean>) [source]
Return the intensity profile of an image measured along a scan line.
imagendarray, shape (M, N[, C]) The image, either grayscale (2D array) or multichannel (3D array, where the final axis contains the channel information).
srcarray_like, shape (2,) The coordinates of the start point of the scan line.
dstarray_like, shape (2,) The coordinates of the end point of the scan line. The destination point is included in the profile, in contrast to standard numpy indexing.
linewidthint, optional Width of the scan, perpendicular to the line
orderint in {0, 1, 2, 3, 4, 5}, optional The order of the spline interpolation, default is 0 if image.dtype is bool and 1 otherwise. The order has to be in the range 0-5. See skimage.transform.warp for detail.
mode{‘constant’, ‘nearest’, ‘reflect’, ‘mirror’, ‘wrap’}, optional How to compute any values falling outside of the image.
cvalfloat, optional If mode is ‘constant’, what constant value to use outside the image.
reduce_funccallable, optional Function used to calculate the aggregation of pixel values perpendicular to the profile_line direction when linewidth > 1. If set to None the unreduced array will be returned.
return_valuearray The intensity profile along the scan line. The length of the profile is the ceil of the computed length of the scan line.
>>> x = np.array([[1, 1, 1, 2, 2, 2]])
>>> img = np.vstack([np.zeros_like(x), x, x, x, np.zeros_like(x)])
>>> img
array([[0, 0, 0, 0, 0, 0],
[1, 1, 1, 2, 2, 2],
[1, 1, 1, 2, 2, 2],
[1, 1, 1, 2, 2, 2],
[0, 0, 0, 0, 0, 0]])
>>> profile_line(img, (2, 1), (2, 4))
array([1., 1., 2., 2.])
>>> profile_line(img, (1, 0), (1, 6), cval=4)
array([1., 1., 1., 2., 2., 2., 2.])
The destination point is included in the profile, in contrast to standard numpy indexing. For example:
>>> profile_line(img, (1, 0), (1, 6)) # The final point is out of bounds array([1., 1., 1., 2., 2., 2., 2.]) >>> profile_line(img, (1, 0), (1, 5)) # This accesses the full first row array([1., 1., 1., 2., 2., 2.])
For different reduce_func inputs:
>>> profile_line(img, (1, 0), (1, 3), linewidth=3, reduce_func=np.mean) array([0.66666667, 0.66666667, 0.66666667, 1.33333333]) >>> profile_line(img, (1, 0), (1, 3), linewidth=3, reduce_func=np.max) array([1, 1, 1, 2]) >>> profile_line(img, (1, 0), (1, 3), linewidth=3, reduce_func=np.sum) array([2, 2, 2, 4])
The unreduced array will be returned when reduce_func is None or when reduce_func acts on each pixel value individually.
>>> profile_line(img, (1, 2), (4, 2), linewidth=3, order=0,
... reduce_func=None)
array([[1, 1, 2],
[1, 1, 2],
[1, 1, 2],
[0, 0, 0]])
>>> profile_line(img, (1, 0), (1, 3), linewidth=3, reduce_func=np.sqrt)
array([[1. , 1. , 0. ],
[1. , 1. , 0. ],
[1. , 1. , 0. ],
[1.41421356, 1.41421356, 0. ]])
skimage.measure.ransac(data, model_class, min_samples, residual_threshold, is_data_valid=None, is_model_valid=None, max_trials=100, stop_sample_num=inf, stop_residuals_sum=0, stop_probability=1, rng=None, initial_inliers=None) [source]
Fit a model to data with the RANSAC (random sample consensus) algorithm.
RANSAC is an iterative algorithm for the robust estimation of parameters from a subset of inliers from the complete data set. Each iteration performs the following tasks:
min_samples random samples from the original data and check whether the set of data is valid (see is_data_valid).model_cls.estimate(*data[random_subset]) and check whether the estimated model is valid (see is_model_valid).model_cls.residuals(*data)) - all data samples with residuals smaller than the residual_threshold are considered as inliers.These steps are performed either a maximum number of times or until one of the special stop criteria are met. The final model is estimated using all inlier samples of the previously determined best model.
data[list, tuple of] (N, …) array Data set to which the model is fitted, where N is the number of data points and the remaining dimension are depending on model requirements. If the model class requires multiple input data arrays (e.g. source and destination coordinates of skimage.transform.AffineTransform), they can be optionally passed as tuple or list. Note, that in this case the functions estimate(*data), residuals(*data), is_model_valid(model, *random_data) and is_data_valid(*random_data) must all take each data array as separate arguments.
model_classobject Object with the following object methods:
success = estimate(*data)residuals(*data)where success indicates whether the model estimation succeeded (True or None for success, False for failure).
min_samplesint in range (0, N) The minimum number of data points to fit a model to.
residual_thresholdfloat larger than 0 Maximum distance for a data point to be classified as an inlier.
is_data_validfunction, optional This function is called with the randomly selected data before the model is fitted to it: is_data_valid(*random_data).
is_model_validfunction, optional This function is called with the estimated model and the randomly selected data: is_model_valid(model, *random_data), .
max_trialsint, optional Maximum number of iterations for random sample selection.
stop_sample_numint, optional Stop iteration if at least this number of inliers are found.
stop_residuals_sumfloat, optional Stop iteration if sum of residuals is less than or equal to this threshold.
stop_probabilityfloat in range [0, 1], optional RANSAC iteration stops if at least one outlier-free set of the training data is sampled with probability >= stop_probability, depending on the current best model’s inlier ratio and the number of trials. This requires to generate at least N samples (trials):
N >= log(1 - probability) / log(1 - e**m)
where the probability (confidence) is typically set to a high value such as 0.99, e is the current fraction of inliers w.r.t. the total number of samples, and m is the min_samples value.
rng{numpy.random.Generator, int}, optional Pseudo-random number generator. By default, a PCG64 generator is used (see numpy.random.default_rng()). If rng is an int, it is used to seed the generator.
initial_inliersarray-like of bool, shape (N,), optional Initial samples selection for model estimation
modelobject Best model with largest consensus set.
inliers(N,) array Boolean mask of inliers classified as True.
“RANSAC”, Wikipedia, https://en.wikipedia.org/wiki/RANSAC
Generate ellipse data without tilt and add noise:
>>> t = np.linspace(0, 2 * np.pi, 50) >>> xc, yc = 20, 30 >>> a, b = 5, 10 >>> x = xc + a * np.cos(t) >>> y = yc + b * np.sin(t) >>> data = np.column_stack([x, y]) >>> rng = np.random.default_rng(203560) # do not copy this value >>> data += rng.normal(size=data.shape)
Add some faulty data:
>>> data[0] = (100, 100) >>> data[1] = (110, 120) >>> data[2] = (120, 130) >>> data[3] = (140, 130)
Estimate ellipse model using all available data:
>>> model = EllipseModel() >>> model.estimate(data) True >>> np.round(model.params) array([ 72., 75., 77., 14., 1.])
Estimate ellipse model using RANSAC:
>>> ransac_model, inliers = ransac(data, EllipseModel, 20, 3, max_trials=50)
>>> abs(np.round(ransac_model.params))
array([20., 30., 10., 6., 2.])
>>> inliers
array([False, False, False, False, True, True, True, True, True,
True, True, True, True, True, True, True, True, True,
True, True, True, True, True, True, True, True, True,
True, True, True, True, True, True, True, True, True,
True, True, True, True, True, True, True, True, True,
True, True, True, True, True], dtype=bool)
>>> sum(inliers) > 40
True
RANSAC can be used to robustly estimate a geometric transformation. In this section, we also show how to use a proportion of the total samples, rather than an absolute number.
>>> from skimage.transform import SimilarityTransform
>>> rng = np.random.default_rng()
>>> src = 100 * rng.random((50, 2))
>>> model0 = SimilarityTransform(scale=0.5, rotation=1,
... translation=(10, 20))
>>> dst = model0(src)
>>> dst[0] = (10000, 10000)
>>> dst[1] = (-100, 100)
>>> dst[2] = (50, 50)
>>> ratio = 0.5 # use half of the samples
>>> min_samples = int(ratio * len(src))
>>> model, inliers = ransac(
... (src, dst),
... SimilarityTransform,
... min_samples,
... 10,
... initial_inliers=np.ones(len(src), dtype=bool),
... )
>>> inliers
array([False, False, False, True, True, True, True, True, True,
True, True, True, True, True, True, True, True, True,
True, True, True, True, True, True, True, True, True,
True, True, True, True, True, True, True, True, True,
True, True, True, True, True, True, True, True, True,
True, True, True, True, True])
skimage.measure.regionprops(label_image, intensity_image=None, cache=True, *, extra_properties=None, spacing=None, offset=None) [source]
Measure properties of labeled image regions.
label_image(M, N[, P]) ndarray Labeled input image. Labels with value 0 are ignored.
Changed in version 0.14.1: Previously, label_image was processed by numpy.squeeze and so any number of singleton dimensions was allowed. This resulted in inconsistent handling of images with singleton dimensions. To recover the old behaviour, use regionprops(np.squeeze(label_image), ...).
intensity_image(M, N[, P][, C]) ndarray, optional Intensity (i.e., input) image with same size as labeled image, plus optionally an extra dimension for multichannel data. Currently, this extra channel dimension, if present, must be the last axis. Default is None.
Changed in version 0.18.0: The ability to provide an extra dimension for channels was added.
cachebool, optional Determine whether to cache calculated properties. The computation is much faster for cached properties, whereas the memory consumption increases.
extra_propertiesIterable of callables Add extra property computation functions that are not included with skimage. The name of the property is derived from the function name, the dtype is inferred by calling the function on a small sample. If the name of an extra property clashes with the name of an existing property the extra property will not be visible and a UserWarning is issued. A property computation function must take a region mask as its first argument. If the property requires an intensity image, it must accept the intensity image as the second argument.
The pixel spacing along each axis of the image.
offsetarray-like of int, shape (label_image.ndim,), optional Coordinates of the origin (“top-left” corner) of the label image. Normally this is ([0, ]0, 0), but it might be different if one wants to obtain regionprops of subvolumes within a larger volume.
propertieslist of RegionProperties Each item describes one labeled region, and can be accessed using the attributes listed below.
See also
The following properties can be accessed as attributes or keys:
areafloat Area of the region i.e. number of pixels of the region scaled by pixel-area.
area_bboxfloat Area of the bounding box i.e. number of pixels of bounding box scaled by pixel-area.
area_convexfloat Area of the convex hull image, which is the smallest convex polygon that encloses the region.
area_filledfloat Area of the region with all the holes filled in.
axis_major_lengthfloat The length of the major axis of the ellipse that has the same normalized second central moments as the region.
axis_minor_lengthfloat The length of the minor axis of the ellipse that has the same normalized second central moments as the region.
bboxtuple Bounding box (min_row, min_col, max_row, max_col). Pixels belonging to the bounding box are in the half-open interval [min_row; max_row) and [min_col; max_col).
centroidarray Centroid coordinate tuple (row, col).
centroid_localarray Centroid coordinate tuple (row, col), relative to region bounding box.
centroid_weightedarray Centroid coordinate tuple (row, col) weighted with intensity image.
centroid_weighted_localarray Centroid coordinate tuple (row, col), relative to region bounding box, weighted with intensity image.
coords_scaled(K, 2) ndarray Coordinate list (row, col) of the region scaled by spacing.
coords(K, 2) ndarray Coordinate list (row, col) of the region.
eccentricityfloat Eccentricity of the ellipse that has the same second-moments as the region. The eccentricity is the ratio of the focal distance (distance between focal points) over the major axis length. The value is in the interval [0, 1). When it is 0, the ellipse becomes a circle.
equivalent_diameter_areafloat The diameter of a circle with the same area as the region.
euler_numberint Euler characteristic of the set of non-zero pixels. Computed as number of connected components subtracted by number of holes (input.ndim connectivity). In 3D, number of connected components plus number of holes subtracted by number of tunnels.
extentfloat Ratio of pixels in the region to pixels in the total bounding box. Computed as area / (rows * cols)
feret_diameter_maxfloat Maximum Feret’s diameter computed as the longest distance between points around a region’s convex hull contour as determined by find_contours. [5]
image(H, J) ndarray Sliced binary region image which has the same size as bounding box.
image_convex(H, J) ndarray Binary convex hull image which has the same size as bounding box.
image_filled(H, J) ndarray Binary region image with filled holes which has the same size as bounding box.
image_intensityndarray Image inside region bounding box.
inertia_tensorndarray Inertia tensor of the region for the rotation around its mass.
inertia_tensor_eigvalstuple The eigenvalues of the inertia tensor in decreasing order.
intensity_maxfloat Value with the greatest intensity in the region.
intensity_meanfloat Value with the mean intensity in the region.
intensity_minfloat Value with the least intensity in the region.
intensity_stdfloat Standard deviation of the intensity in the region.
labelint The label in the labeled input image.
moments(3, 3) ndarray Spatial moments up to 3rd order:
m_ij = sum{ array(row, col) * row^i * col^j }
where the sum is over the row, col coordinates of the region.
moments_central(3, 3) ndarray Central moments (translation invariant) up to 3rd order:
mu_ij = sum{ array(row, col) * (row - row_c)^i * (col - col_c)^j }
where the sum is over the row, col coordinates of the region, and row_c and col_c are the coordinates of the region’s centroid.
moments_hutuple Hu moments (translation, scale and rotation invariant).
moments_normalized(3, 3) ndarray Normalized moments (translation and scale invariant) up to 3rd order:
nu_ij = mu_ij / m_00^[(i+j)/2 + 1]
where m_00 is the zeroth spatial moment.
moments_weighted(3, 3) ndarray Spatial moments of intensity image up to 3rd order:
wm_ij = sum{ array(row, col) * row^i * col^j }
where the sum is over the row, col coordinates of the region.
moments_weighted_central(3, 3) ndarray Central moments (translation invariant) of intensity image up to 3rd order:
wmu_ij = sum{ array(row, col) * (row - row_c)^i * (col - col_c)^j }
where the sum is over the row, col coordinates of the region, and row_c and col_c are the coordinates of the region’s weighted centroid.
moments_weighted_hutuple Hu moments (translation, scale and rotation invariant) of intensity image.
moments_weighted_normalized(3, 3) ndarray Normalized moments (translation and scale invariant) of intensity image up to 3rd order:
wnu_ij = wmu_ij / wm_00^[(i+j)/2 + 1]
where wm_00 is the zeroth spatial moment (intensity-weighted area).
num_pixelsint Number of foreground pixels.
orientationfloat Angle between the 0th axis (rows) and the major axis of the ellipse that has the same second moments as the region, ranging from -pi/2 to pi/2 counter-clockwise.
perimeterfloat Perimeter of object which approximates the contour as a line through the centers of border pixels using a 4-connectivity.
perimeter_croftonfloat Perimeter of object approximated by the Crofton formula in 4 directions.
slicetuple of slices A slice to extract the object from the source image.
solidityfloat Ratio of pixels in the region to pixels of the convex hull image.
Each region also supports iteration, so that you can do:
for prop in region:
print(prop, region[prop])
Wilhelm Burger, Mark Burge. Principles of Digital Image Processing: Core Algorithms. Springer-Verlag, London, 2009.
B. Jähne. Digital Image Processing. Springer-Verlag, Berlin-Heidelberg, 6. edition, 2005.
T. H. Reiss. Recognizing Planar Objects Using Invariant Image Features, from Lecture notes in computer science, p. 676. Springer, Berlin, 1993.
W. Pabst, E. Gregorová. Characterization of particles and particle systems, pp. 27-28. ICT Prague, 2007. https://old.vscht.cz/sil/keramika/Characterization_of_particles/CPPS%20_English%20version_.pdf
>>> from skimage import data, util >>> from skimage.measure import label, regionprops >>> img = util.img_as_ubyte(data.coins()) > 110 >>> label_img = label(img, connectivity=img.ndim) >>> props = regionprops(label_img) >>> # centroid of first labeled object >>> props[0].centroid (22.72987986048314, 81.91228523446583) >>> # centroid of first labeled object >>> props[0]['centroid'] (22.72987986048314, 81.91228523446583)
Add custom measurements by passing functions as extra_properties
>>> from skimage import data, util >>> from skimage.measure import label, regionprops >>> import numpy as np >>> img = util.img_as_ubyte(data.coins()) > 110 >>> label_img = label(img, connectivity=img.ndim) >>> def pixelcount(regionmask): ... return np.sum(regionmask) >>> props = regionprops(label_img, extra_properties=(pixelcount,)) >>> props[0].pixelcount 7741 >>> props[1]['pixelcount'] 42
skimage.measure.regionprops_table(label_image, intensity_image=None, properties=('label', 'bbox'), *, cache=True, separator='-', extra_properties=None, spacing=None) [source]
Compute image properties and return them as a pandas-compatible table.
The table is a dictionary mapping column names to value arrays. See Notes section below for details.
Added in version 0.16.
label_image(M, N[, P]) ndarray Labeled input image. Labels with value 0 are ignored.
intensity_image(M, N[, P][, C]) ndarray, optional Intensity (i.e., input) image with same size as labeled image, plus optionally an extra dimension for multichannel data. The channel dimension, if present, must be the last axis. Default is None.
Changed in version 0.18.0: The ability to provide an extra dimension for channels was added.
propertiestuple or list of str, optional Properties that will be included in the resulting dictionary For a list of available properties, please see regionprops(). Users should remember to add “label” to keep track of region identities.
cachebool, optional Determine whether to cache calculated properties. The computation is much faster for cached properties, whereas the memory consumption increases.
separatorstr, optional For non-scalar properties not listed in OBJECT_COLUMNS, each element will appear in its own column, with the index of that element separated from the property name by this separator. For example, the inertia tensor of a 2D region will appear in four columns: inertia_tensor-0-0, inertia_tensor-0-1, inertia_tensor-1-0, and inertia_tensor-1-1 (where the separator is -).
Object columns are those that cannot be split in this way because the number of columns would change depending on the object. For example, image and coords.
extra_propertiesIterable of callables Add extra property computation functions that are not included with skimage. The name of the property is derived from the function name, the dtype is inferred by calling the function on a small sample. If the name of an extra property clashes with the name of an existing property the extra property will not be visible and a UserWarning is issued. A property computation function must take a region mask as its first argument. If the property requires an intensity image, it must accept the intensity image as the second argument.
The pixel spacing along each axis of the image.
out_dictdict Dictionary mapping property names to an array of values of that property, one value per region. This dictionary can be used as input to pandas DataFrame to map property names to columns in the frame and regions to rows. If the image has no regions, the arrays will have length 0, but the correct type.
Each column contains either a scalar property, an object property, or an element in a multidimensional array.
Properties with scalar values for each region, such as “eccentricity”, will appear as a float or int array with that property name as key.
Multidimensional properties of fixed size for a given image dimension, such as “centroid” (every centroid will have three elements in a 3D image, no matter the region size), will be split into that many columns, with the name {property_name}{separator}{element_num} (for 1D properties), {property_name}{separator}{elem_num0}{separator}{elem_num1} (for 2D properties), and so on.
For multidimensional properties that don’t have a fixed size, such as “image” (the image of a region varies in size depending on the region size), an object array will be used, with the corresponding property name as the key.
>>> from skimage import data, util, measure
>>> image = data.coins()
>>> label_image = measure.label(image > 110, connectivity=image.ndim)
>>> props = measure.regionprops_table(label_image, image,
... properties=['label', 'inertia_tensor',
... 'inertia_tensor_eigvals'])
>>> props
{'label': array([ 1, 2, ...]), ...
'inertia_tensor-0-0': array([ 4.012...e+03, 8.51..., ...]), ...
...,
'inertia_tensor_eigvals-1': array([ 2.67...e+02, 2.83..., ...])}
The resulting dictionary can be directly passed to pandas, if installed, to obtain a clean DataFrame:
>>> import pandas as pd >>> data = pd.DataFrame(props) >>> data.head() label inertia_tensor-0-0 ... inertia_tensor_eigvals-1 0 1 4012.909888 ... 267.065503 1 2 8.514739 ... 2.834806 2 3 0.666667 ... 0.000000 3 4 0.000000 ... 0.000000 4 5 0.222222 ... 0.111111
[5 rows x 7 columns]
If we want to measure a feature that does not come as a built-in property, we can define custom functions and pass them as extra_properties. For example, we can create a custom function that measures the intensity quartiles in a region:
>>> from skimage import data, util, measure
>>> import numpy as np
>>> def quartiles(regionmask, intensity):
... return np.percentile(intensity[regionmask], q=(25, 50, 75))
>>>
>>> image = data.coins()
>>> label_image = measure.label(image > 110, connectivity=image.ndim)
>>> props = measure.regionprops_table(label_image, intensity_image=image,
... properties=('label',),
... extra_properties=(quartiles,))
>>> import pandas as pd
>>> pd.DataFrame(props).head()
label quartiles-0 quartiles-1 quartiles-2
0 1 117.00 123.0 130.0
1 2 111.25 112.0 114.0
2 3 111.00 111.0 111.0
3 4 111.00 111.5 112.5
4 5 112.50 113.0 114.0
Explore and visualize region properties with pandas
Measure fluorescence intensity at the nuclear envelope
skimage.measure.shannon_entropy(image, base=2) [source]
Calculate the Shannon entropy of an image.
The Shannon entropy is defined as S = -sum(pk * log(pk)), where pk are frequency/probability of pixels of value k.
image(M, N) ndarray Grayscale input image.
basefloat, optional The logarithmic base to use.
entropyfloat The returned value is measured in bits or shannon (Sh) for base=2, natural unit (nat) for base=np.e and hartley (Hart) for base=10.
>>> from skimage import data >>> from skimage.measure import shannon_entropy >>> shannon_entropy(data.camera()) 7.231695011055706
skimage.measure.subdivide_polygon(coords, degree=2, preserve_ends=False) [source]
Subdivision of polygonal curves using B-Splines.
Note that the resulting curve is always within the convex hull of the original polygon. Circular polygons stay closed after subdivision.
coords(K, 2) array Coordinate array.
degree{1, 2, 3, 4, 5, 6, 7}, optional Degree of B-Spline. Default is 2.
preserve_endsbool, optional Preserve first and last coordinate of non-circular polygon. Default is False.
coords(L, 2) array Subdivided coordinate array.
class skimage.measure.CircleModel [source]
Bases: BaseModel
Total least squares estimator for 2D circles.
The functional model of the circle is:
r**2 = (x - xc)**2 + (y - yc)**2
This estimator minimizes the squared distances from all points to the circle:
min{ sum((r - sqrt((x_i - xc)**2 + (y_i - yc)**2))**2) }
A minimum number of 3 points is required to solve for the parameters.
paramstuple Circle model parameters in the following order xc, yc, r.
The estimation is carried out using a 2D version of the spherical estimation given in [1].
Jekel, Charles F. Obtaining non-linear orthotropic material models for pvc-coated polyester via inverse bubble inflation. Thesis (MEng), Stellenbosch University, 2016. Appendix A, pp. 83-87. https://hdl.handle.net/10019.1/98627
>>> t = np.linspace(0, 2 * np.pi, 25)
>>> xy = CircleModel().predict_xy(t, params=(2, 3, 4))
>>> model = CircleModel()
>>> model.estimate(xy)
True
>>> tuple(np.round(model.params, 5))
(2.0, 3.0, 4.0)
>>> res = model.residuals(xy)
>>> np.abs(np.round(res, 9))
array([0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0.,
0., 0., 0., 0., 0., 0., 0., 0.])
__init__() [source]
estimate(data) [source]
Estimate circle model from data using total least squares.
data(N, 2) array N points with (x, y) coordinates, respectively.
successbool True, if model estimation succeeds.
predict_xy(t, params=None) [source]
Predict x- and y-coordinates using the estimated model.
tarray Angles in circle in radians. Angles start to count from positive x-axis to positive y-axis in a right-handed system.
params(3,) array, optional Optional custom parameter set.
xy(…, 2) array Predicted x- and y-coordinates.
residuals(data) [source]
Determine residuals of data to model.
For each point the shortest distance to the circle is returned.
data(N, 2) array N points with (x, y) coordinates, respectively.
residuals(N,) array Residual for each data point.
class skimage.measure.EllipseModel [source]
Bases: BaseModel
Total least squares estimator for 2D ellipses.
The functional model of the ellipse is:
xt = xc + a*cos(theta)*cos(t) - b*sin(theta)*sin(t) yt = yc + a*sin(theta)*cos(t) + b*cos(theta)*sin(t) d = sqrt((x - xt)**2 + (y - yt)**2)
where (xt, yt) is the closest point on the ellipse to (x, y). Thus d is the shortest distance from the point to the ellipse.
The estimator is based on a least squares minimization. The optimal solution is computed directly, no iterations are required. This leads to a simple, stable and robust fitting method.
The params attribute contains the parameters in the following order:
xc, yc, a, b, theta
paramstuple Ellipse model parameters in the following order xc, yc, a, b, theta.
>>> xy = EllipseModel().predict_xy(np.linspace(0, 2 * np.pi, 25),
... params=(10, 15, 8, 4, np.deg2rad(30)))
>>> ellipse = EllipseModel()
>>> ellipse.estimate(xy)
True
>>> np.round(ellipse.params, 2)
array([10. , 15. , 8. , 4. , 0.52])
>>> np.round(abs(ellipse.residuals(xy)), 5)
array([0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0.,
0., 0., 0., 0., 0., 0., 0., 0.])
__init__() [source]
estimate(data) [source]
Estimate ellipse model from data using total least squares.
data(N, 2) array N points with (x, y) coordinates, respectively.
successbool True, if model estimation succeeds.
Halir, R.; Flusser, J. “Numerically stable direct least squares fitting of ellipses”. In Proc. 6th International Conference in Central Europe on Computer Graphics and Visualization. WSCG (Vol. 98, pp. 125-132).
predict_xy(t, params=None) [source]
Predict x- and y-coordinates using the estimated model.
tarray Angles in circle in radians. Angles start to count from positive x-axis to positive y-axis in a right-handed system.
params(5,) array, optional Optional custom parameter set.
xy(…, 2) array Predicted x- and y-coordinates.
residuals(data) [source]
Determine residuals of data to model.
For each point the shortest distance to the ellipse is returned.
data(N, 2) array N points with (x, y) coordinates, respectively.
residuals(N,) array Residual for each data point.
class skimage.measure.LineModelND [source]
Bases: BaseModel
Total least squares estimator for N-dimensional lines.
In contrast to ordinary least squares line estimation, this estimator minimizes the orthogonal distances of points to the estimated line.
Lines are defined by a point (origin) and a unit vector (direction) according to the following vector equation:
X = origin + lambda * direction
paramstuple Line model parameters in the following order origin, direction.
>>> x = np.linspace(1, 2, 25)
>>> y = 1.5 * x + 3
>>> lm = LineModelND()
>>> lm.estimate(np.stack([x, y], axis=-1))
True
>>> tuple(np.round(lm.params, 5))
(array([1.5 , 5.25]), array([0.5547 , 0.83205]))
>>> res = lm.residuals(np.stack([x, y], axis=-1))
>>> np.abs(np.round(res, 9))
array([0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0., 0.,
0., 0., 0., 0., 0., 0., 0., 0.])
>>> np.round(lm.predict_y(x[:5]), 3)
array([4.5 , 4.562, 4.625, 4.688, 4.75 ])
>>> np.round(lm.predict_x(y[:5]), 3)
array([1. , 1.042, 1.083, 1.125, 1.167])
__init__() [source]
estimate(data) [source]
Estimate line model from data.
This minimizes the sum of shortest (orthogonal) distances from the given data points to the estimated line.
data(N, dim) array N points in a space of dimensionality dim >= 2.
successbool True, if model estimation succeeds.
predict(x, axis=0, params=None) [source]
Predict intersection of the estimated line model with a hyperplane orthogonal to a given axis.
x(n, 1) array Coordinates along an axis.
axisint Axis orthogonal to the hyperplane intersecting the line.
params(2,) array, optional Optional custom parameter set in the form (origin, direction).
data(n, m) array Predicted coordinates.
If the line is parallel to the given axis.
predict_x(y, params=None) [source]
Predict x-coordinates for 2D lines using the estimated model.
Alias for:
predict(y, axis=1)[:, 0]
yarray y-coordinates.
params(2,) array, optional Optional custom parameter set in the form (origin, direction).
xarray Predicted x-coordinates.
predict_y(x, params=None) [source]
Predict y-coordinates for 2D lines using the estimated model.
Alias for:
predict(x, axis=0)[:, 1]
xarray x-coordinates.
params(2,) array, optional Optional custom parameter set in the form (origin, direction).
yarray Predicted y-coordinates.
residuals(data, params=None) [source]
Determine residuals of data to model.
For each point, the shortest (orthogonal) distance to the line is returned. It is obtained by projecting the data onto the line.
data(N, dim) array N points in a space of dimension dim.
params(2,) array, optional Optional custom parameter set in the form (origin, direction).
residuals(N,) array Residual for each data point.
© 2019 the scikit-image team
Licensed under the BSD 3-clause License.
https://scikit-image.org/docs/0.25.x/api/skimage.measure.html